FastQCFastQC Report
Mon 6 Jun 2016
ERR523161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8237350
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT481160.58411989292673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273870.3324734289547002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218050.2647089173095717No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT141390.1716450071928472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123350.1497447601473775No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC103950.1261934966949322No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG309450.029.0662171
TACATGG315350.028.0879652
ACATGGG329600.026.3613823
CATGGGG238700.024.5993564
ATGGGGG141500.022.394075
GAGTACT225750.021.08182512-13
GTACTTT242700.019.41762214-15
GAGTACA236000.018.767351
AGAGTAC364100.018.67660510-11
AGTACTT234800.017.9982212-13
AGTACAT235550.017.4445822
TATACTG35500.016.951625
CATGGGA109750.016.7060494
ACTTTTT295000.016.14942216-17
TGGGGGG138200.015.5373046
GTATATA38050.015.3225511
TACTTTT278100.015.07898414-15
ATGGGGA92350.014.7126385
GTACAGG56050.014.5122421
GTATAGA28750.014.228051
Produced by FastQC (version 0.11.5)