FastQCFastQC Report
Mon 6 Jun 2016
ERR523161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8237350
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT492680.5981049730799347No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255730.31045178364401177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212360.2578013560186225No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT140430.17047958384674683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117420.14254584301990325No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC110670.13435146011763494No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC104710.12711612351059504No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG316850.027.802631
TACATGG319450.027.3097932
ACATGGG328450.025.6401043
CATGGGG234150.024.1045994
ATGGGGG142500.022.3596945
GAGTACT234350.020.76387412-13
TATACCG6200.019.7075525
GTACTTT249300.019.31693614-15
AGAGTAC379300.018.20829610-11
GAGTACA241400.018.0416181
AGTACTT242500.017.76862112-13
TGGGGGG130450.017.0399426
AGTACAT237500.016.9811462
ACTTTTT300650.016.09257116-17
CATGGGA111250.015.7565044
TACTTTT283450.015.17376114-15
GTATAGG23700.015.082281
GTATATA38100.015.0604051
TATACTG37350.014.9729515
TACCTGG54950.014.5420132
Produced by FastQC (version 0.11.5)