FastQCFastQC Report
Mon 6 Jun 2016
ERR523160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17242789
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT596250.34579672696800967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488030.2830342585529522No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT390410.2264192875062149No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278430.16147619738314956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG167700.028.60012
GTACCTG201350.024.831891
GTACATG845950.021.8567941
TACATGG863050.021.0796412
GAGTACT482400.020.95710612-13
CATGGGG513800.020.661694
ACCTGGG228700.020.3548073
ACATGGG882150.020.1007373
GTATCAA1180950.019.5320171
GTACTTT533300.018.83936314-15
AGTACTT510000.018.46395712-13
ATGGGGG283200.018.0446955
TCAACGC1299350.017.5525864
CAACGCA1304950.017.4691435
ATCAACG1324650.017.2233493
TATCAAC1328300.017.197362
AACGCAG1330450.017.186496
AGAGTAC984800.016.54819110-11
GGTATCA938350.015.9350621
ACTTTTT646300.015.93098816-17
Produced by FastQC (version 0.11.5)