FastQCFastQC Report
Mon 6 Jun 2016
ERR523160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17242789
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT536920.3113881402828742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT476590.2763996010158217No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT401750.23299594978515367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276190.16017710359965548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG170350.028.5812532
GTACCTG206400.024.3987541
ACCTGGG226150.020.904263
GAGTACT498850.020.52273812-13
GTACATG855250.020.2981511
CATGGGG493050.020.1773134
TACATGG867950.019.7309172
ACATGGG883100.018.8589383
GTACTTT545000.018.63627214-15
AGTACTT525050.017.91405112-13
ATGGGGG275950.017.3871145
GTATCAA1197850.017.1383551
GGTATCA983650.016.4248141
ACTTTTT647450.015.867166516-17
TACTTTT601250.015.5286714-15
AGAGTAC1024750.015.44659110-11
TATAACG13750.015.3806072
TCAACGC1322400.015.3480714
CAACGCA1327200.015.3102215
TATCAAC1351400.015.1066982
Produced by FastQC (version 0.11.5)