FastQCFastQC Report
Mon 6 Jun 2016
ERR523159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9266165
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328680.3547098502994497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269530.290875459264971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140290.15140028264120053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211100.040.589531
GTATCAA347850.036.3405271
TCAACGC412050.030.2551754
ATCAACG414400.030.027513
CAACGCA416950.029.7729765
TATCAAC427650.029.2845782
AACGCAG428850.029.0796186
GTACATG405100.026.6888981
TACCTGG81250.026.2777842
TACATGG411850.026.0118312
ACGCAGA488300.025.3050297
ACATGGG416350.024.8153693
CGCAGAG501650.024.6215728
TATAACG7550.024.2925952
CATGGGG266550.023.9056434
ATGGGGG166400.022.493765
GCAGAGT547100.022.2662859
GAGTACT268750.021.76341812-13
ACCTGGG100500.020.5422343
GTACTTT289700.019.99575814-15
Produced by FastQC (version 0.11.5)