FastQCFastQC Report
Mon 6 Jun 2016
ERR523159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9266165
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308150.3325539745946678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264990.28597591344423506No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT181650.19603579258517415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138850.1498462416760332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA214900.040.5331951
GTATCAA344700.034.4120141
TCAACGC408700.028.6125534
ATCAACG411250.028.4465683
CAACGCA415450.028.0912555
TATCAAC425200.027.6242752
AACGCAG426650.027.4510566
TACCTGG85300.025.2368052
GTACATG397800.024.970971
TACATGG404400.024.2798042
ACGCAGA490050.023.9093077
ACATGGG407750.023.3076273
CGCAGAG502700.023.2321038
CATGGGG262200.022.622344
TAACGCA8250.022.2188474
TATAACG9150.022.0884512
ATGGGGG153950.022.0430015
ACCTGGG98200.021.1554183
GAGTACT265150.021.07514612-13
GCAGAGT551250.021.0495839
Produced by FastQC (version 0.11.5)