FastQCFastQC Report
Mon 6 Jun 2016
ERR523158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12757638
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410430.3217131572474466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339050.26576236133992825No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT299580.2348240324737228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192910.1512113762751381No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG123200.028.8559952
GTACCTG145850.025.5407871
ACCTGGG148050.023.0914363
GTACATG553300.021.387771
CATGGGG295500.020.796824
GAGTACT365500.020.55793612-13
TACATGG561500.020.4847972
ACATGGG565350.019.7048263
GTATCAA819100.018.8172021
GTACTTT408700.018.414314-15
AGTACTT392800.017.78926312-13
ATGGGGG168800.017.4917625
TCAACGC897700.017.0647034
CAACGCA900450.017.0217575
ATCAACG907300.016.8703863
AACGCAG916250.016.7561116
TATCAAC923700.016.5504952
AGAGTAC679400.016.3766510-11
GGTATCA655750.016.2531361
GTATAGG36250.015.8295051
Produced by FastQC (version 0.11.5)