FastQCFastQC Report
Mon 6 Jun 2016
ERR523158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12757638
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365110.2861893400643599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327220.25648948496579066No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT316750.24828263664480837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190040.14896174354531772No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG116650.030.0970552
GTACCTG139150.025.9174731
ACCTGGG152150.022.639443
GTACATG562950.020.7640381
GAGTACT359900.020.38259112-13
CATGGGG288050.020.0990944
TACATGG569100.020.0020122
ACATGGG572250.019.1421243
GTACTTT394550.018.44662314-15
AGTACTT387100.017.58421112-13
ATGGGGG162300.017.0251435
GTATCAA849250.016.726141
AGAGTAC681950.015.49255110-11
GGTATCA698700.015.4108031
TCAACGC915450.015.2203174
CAACGCA919050.015.1759795
ACTTTTT492750.015.06396816-17
AACGCAG934050.014.9775466
ATCAACG931250.014.9469433
TATCAAC952400.014.69580652
Produced by FastQC (version 0.11.5)