FastQCFastQC Report
Mon 6 Jun 2016
ERR523157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5588454
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163210.2920485701412233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133250.23843803670925806No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT100350.179566656538642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65750.11765329015860201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG57850.028.2075862
TATAACG4850.025.209922
GTACATG229100.024.7955591
TACATGG233850.023.99072
ACCTGGG68050.023.0809843
ACATGGG235750.022.959353
CATGGGG150800.022.2031524
GTATCAA307900.022.1610221
GGTATCA234050.021.6390971
GTACCTG78400.021.353351
GAGTACT145700.020.6131312-13
TCAACGC339350.019.8995154
CAACGCA343200.019.7166865
ATCAACG346250.019.5437073
AACGCAG350750.019.4045966
TATCAAC350000.019.3747942
GTACTTT155750.018.98186314-15
CGTATCA14050.018.72961294
ATGGGGG88800.018.640185
GTATAGG15400.018.3215661
Produced by FastQC (version 0.11.5)