FastQCFastQC Report
Mon 6 Jun 2016
ERR523157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5588454
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151500.27109465336925026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133870.2395474669738715No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT101100.18090870927809372No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT100350.179566656538642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68720.12296781900683086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG56800.030.4525782
ACCTGGG66450.025.0383473
TATAACG5050.024.1995092
GTACCTG75750.023.5333821
TAACGCA5300.023.0565784
GGTATCA228200.022.8170641
GTATCAA300200.022.5464361
GTACATG215050.022.3313051
TACATGG220800.021.3726922
GAGTACT154800.020.90385812-13
ACATGGG220350.020.540483
TCAACGC333350.020.1055724
CAACGCA335950.019.9921195
ATCAACG337000.019.7901863
CATGGGG141200.019.7053594
AACGCAG345750.019.4516016
TATCAAC344400.019.4071182
GTACTTT163850.019.3184814-15
ATAACGC6600.018.5151313
CGAGACT15300.018.43118712-13
Produced by FastQC (version 0.11.5)