FastQCFastQC Report
Mon 6 Jun 2016
ERR523156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6143462
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT500780.8151429926643966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249780.40657857084490795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205240.33407873280570466No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT133060.21658797596534335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125970.2050472518589681No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC111310.1811844852299892No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC95040.1547010464132439No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG75080.1222112222717419No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC67070.10917297120092873No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG55500.038.546362
GTACCTG69450.031.3479541
ACCTGGG70350.029.7423343
TATAACG5500.029.065732
GTACATG224400.028.3933681
TACATGG230600.027.0568082
ACATGGG231700.025.9957453
CATGGGG164200.024.481224
ATGGGGG107500.022.8301755
ATAACGC7900.020.8314783
GAGTACT201300.020.53823712-13
AGAGTAC303600.019.7200210-11
CCTGGGG68150.019.4546474
TAACGCA7500.019.4330314
TGGGGGG110050.018.912336
AGTACTT213850.018.78369912-13
TATCACG5650.018.3079282
GTACTTT228550.017.89498314-15
GAGTACA174000.017.1603031
AGTACAT168950.015.9185242
Produced by FastQC (version 0.11.5)