FastQCFastQC Report
Mon 6 Jun 2016
ERR523156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6143462
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT500060.8139710150400539No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228550.3720215083938014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202810.33012330832354786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122260.19900831160020196No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC112980.1839028222197842No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC100370.16337693632678124No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA85620.13936767249475948No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG79900.13005696136803646No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA77610.12632942142394632No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG57000.039.1700672
TATAACG5550.034.7237362
GTACCTG70650.032.225351
TAACGCA6700.030.1611484
ACCTGGG74150.029.914733
GTACATG220700.028.2834911
ATAACGC7350.026.8544453
TACATGG227650.026.8211292
ACATGGG227150.025.778613
CATGGGG160200.024.7297254
ATGGGGG104950.024.269485
CCTGGGG67100.021.5015664
TGGGGGG103800.020.9164646
GAGTACT210900.020.79818712-13
TATCACG6250.019.5536962
AGAGTAC315600.019.47697810-11
GTATAAC18500.019.3244131
AGTACTT223100.018.8179512-13
GTACTTT234750.018.44151714-15
GTATAGG15950.016.2205831
Produced by FastQC (version 0.11.5)