FastQCFastQC Report
Mon 6 Jun 2016
ERR523155_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523155_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5958644
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT427600.717612933412367No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213900.3589742901237261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174580.29298612234595656No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT128030.2148643214798535No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC106180.17819490474678465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98040.16453407855881305No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC88300.14818807769015904No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG70200.11781203911493958No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA65070.10920269779500168No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG43800.025.5468062
GTACATG208200.023.7357711
TACATGG212750.022.8278262
ATAACGC5550.022.0267683
ACATGGG215250.021.7563763
GTACCTG54700.021.2320041
GAGTACT189200.020.53498612-13
CATGGGG134100.019.9146024
TAACGCA6050.019.4283854
AGAGTAC286550.019.41922610-11
GTACTTT203050.018.91362214-15
AGTACTT197800.018.4189812-13
ACCTGGG59350.018.2212223
TATAACG7700.017.706842
GTGTAGC33900.017.476411
ATGGGGG76600.017.2474945
TAGCCCT41900.015.59742454
ACTTTTT252250.015.58751616-17
GAGTACA167900.014.9825061
TATACTG28250.014.9786435
Produced by FastQC (version 0.11.5)