FastQCFastQC Report
Mon 6 Jun 2016
ERR523155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523155_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5958644
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT447220.7505398879342348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197590.33160229072252007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169470.28441034570952717No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT130190.21848930729877467No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC106710.17908436885976073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93470.156864548377114No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC93200.15641142514974885No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA76950.12914011979906836No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG73180.1228131769577105No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA70330.11803020955774501No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG203750.024.028551
TACCTGG44600.023.5025372
TACATGG204800.023.2500692
ACATGGG207100.022.5110683
GTACCTG52000.020.7112621
CATGGGG133200.020.640314
GAGTACT191700.020.5020912-13
GTACTTT204150.018.9612214-15
AGAGTAC287800.018.75244110-11
ATGGGGG72250.017.9523455
AGTACTT199950.017.90451212-13
ATAACGC5600.017.6236483
ACCTGGG56550.017.6184563
TATCACG6250.016.5457862
GAGTACA165200.016.2555071
ACTTTTT248400.015.65659616-17
AGTACAT160200.015.4042842
CATGGGA74500.015.0766954
TATACAG41200.014.4862675
TACTTTT245800.014.37140314-15
Produced by FastQC (version 0.11.5)