FastQCFastQC Report
Mon 6 Jun 2016
ERR523154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4936067
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT540471.0949405670547017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167120.33856914827128565No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT150950.30581027364498903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127060.25741141682234053No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC113200.22933238142837203No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC99990.20257018391363005No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG83250.16865654376247324No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA81210.16452369872613154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80240.1625585714294397No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA77740.1574938103554915No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC71060.14396076876590208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG38850.031.7079322
GTACATG184150.029.1092171
TACATGG186250.028.2482452
ACATGGG189650.027.1476463
CATGGGG137500.025.474574
ATGGGGG90050.025.4277155
GTACCTG50400.025.003551
ATAACGC5200.024.4135743
TAACGCA5350.023.7281134
TATCACG6000.023.5086482
ACCTGGG53550.022.7410853
TATAACG5200.021.7002932
GGGTTAG20500.021.1023771
TGGGGGG93450.020.5630786
GAGTACT143850.019.94097512-13
AGAGTAC229100.019.2048710-11
AGTACTT152400.017.98980912-13
GGTTAGA22250.017.9616642
GTATAGG12850.017.9304161
GTTAGAG22950.017.4143453
Produced by FastQC (version 0.11.5)