FastQCFastQC Report
Mon 6 Jun 2016
ERR523154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523154_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4936067
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT534321.0824812548127891No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149940.303764110171114No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT143900.2915276474164552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119970.24304775441662357No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC115340.23366781690767163No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC100500.20360339517271545No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA83120.16839317618662794No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG82960.16806903147789526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75410.15277345303457185No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG40450.031.9529952
GTACATG178900.028.6289481
TACATGG180300.028.0748442
ACATGGG180250.026.723763
GTACCTG50200.026.1389621
ATGGGGG81200.025.5229915
CATGGGG129200.025.2796634
ATAACGC5550.024.5557963
TAACGCA4950.023.7347034
ACCTGGG54000.023.4973553
GGGTTAG18850.022.7048111
TATCACG5050.021.4059162
TGGGGGG84800.020.9481166
GAGTACT146450.019.21417612-13
AGAGTAC228850.018.78768210-11
AGTACTT154200.018.32470912-13
TATAACG5950.018.1680472
GTACTTT164700.016.9531214-15
GTTAGAG23750.016.819163
GTACACG7850.016.775531
Produced by FastQC (version 0.11.5)