FastQCFastQC Report
Mon 6 Jun 2016
ERR523153_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523153_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7459668
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309150.41442863140826103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253560.33990788866206917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160180.2147280549214791No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131830.17672368260893112No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG118400.054.1361162
GTACCTG140000.046.1282771
ACCTGGG144300.043.799593
CCTGGGG134850.033.440944
TATAACG6950.031.8021142
GGTATCA237200.029.4070321
GTATCAA345250.028.8683551
ATAACGC9350.025.650493
TAACGCA8800.025.1145844
TATCACG6950.025.0357072
TCAACGC404350.024.2238184
CAACGCA407750.024.0333655
TATCAAC413100.023.8149382
ATCAACG413050.023.7150343
AACGCAG418500.023.5698176
GTACATG349900.023.0270421
CTGGGGG86050.022.9514035
TACATGG359350.022.1555352
CATGGGG208700.021.9681244
ACATGGG364250.021.3020553
Produced by FastQC (version 0.11.5)