FastQCFastQC Report
Mon 6 Jun 2016
ERR523153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7459668
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281900.37789885555228464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253590.3399481049290666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160110.2146342169651518No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121400.16274182711616655No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG127050.053.3793562
GTACCTG150700.045.8016171
ACCTGGG151950.044.1018523
CCTGGGG137250.034.0339244
TATAACG7050.033.3320922
GGTATCA257950.029.273731
GTATCAA364500.027.0501021
TATCACG7950.025.4204352
CTGGGGG83000.024.9686815
TAACGCA10050.024.7826524
ATAACGC9850.023.377493
TCAACGC430550.022.6372224
CAACGCA436100.022.3166545
TATCAAC440000.022.206672
ATCAACG439450.022.1680663
AACGCAG447500.021.9056596
GTACACG19400.021.812671
GTACATG352850.020.8141611
TGGGGGG114250.020.483696
GAGTACT273150.019.9000312-13
Produced by FastQC (version 0.11.5)