##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523153_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7459668
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11684715727295	33.0	31.0	34.0	30.0	34.0
2	32.24638844516941	34.0	31.0	34.0	30.0	34.0
3	32.42410238632604	34.0	31.0	34.0	30.0	35.0
4	35.5404725786724	37.0	35.0	37.0	33.0	37.0
5	35.47825680713941	36.0	35.0	37.0	33.0	37.0
6	35.60838082874466	37.0	35.0	37.0	33.0	37.0
7	35.4505691137997	36.0	35.0	37.0	33.0	37.0
8	35.437424158823156	36.0	35.0	37.0	33.0	37.0
9	36.70991738506325	37.0	35.0	39.0	33.0	39.0
10-11	36.51081723744274	37.5	35.0	39.0	32.5	39.0
12-13	36.514220941200065	37.5	35.0	39.0	33.0	39.0
14-15	37.656796455284606	38.0	37.0	41.0	33.0	41.0
16-17	37.46677955372813	38.0	36.5	41.0	32.5	41.0
18-19	37.363474084905654	38.0	36.0	41.0	32.0	41.0
20-21	37.3847704080128	38.0	36.0	40.5	32.5	41.0
22-23	37.223994150946126	38.0	35.5	40.0	32.0	41.0
24-25	37.140183986740425	38.0	36.5	40.0	32.0	41.0
26-27	36.81925448960999	38.0	36.0	40.0	31.5	41.0
28-29	36.75409575868524	38.0	35.5	40.0	31.0	41.0
30-31	36.50078663018247	37.5	35.5	40.0	31.0	41.0
32-33	36.43190990537381	37.5	36.0	40.0	30.5	41.0
34-35	36.189104796620974	37.5	35.0	40.0	30.5	41.0
36-37	35.952963322228285	37.0	35.0	40.0	30.0	41.0
38-39	35.89856237569822	37.0	35.0	40.0	30.0	41.0
40-41	36.07644576139313	37.5	35.0	40.0	30.0	41.0
42-43	36.1033073455816	37.5	35.0	40.0	30.0	41.0
44-45	35.920073989888024	37.0	35.0	40.0	30.0	41.0
46-47	35.78441500345592	37.0	35.0	40.0	28.5	41.0
48-49	35.54828251069618	37.5	34.5	40.0	28.0	41.0
50-51	35.370771058443886	37.5	34.0	40.0	27.5	41.0
52-53	35.35498349256294	37.5	34.0	40.0	27.0	41.0
54-55	35.14956865372561	37.5	34.0	40.0	26.0	41.0
56-57	34.89705533811961	37.0	34.0	39.5	26.0	41.0
58-59	34.7006173733201	36.5	34.0	39.0	26.5	41.0
60-61	34.501286384327024	36.5	33.0	39.0	26.0	41.0
62-63	34.250486147640885	36.0	33.0	39.0	25.0	40.5
64-65	34.049499924125314	36.5	33.0	38.0	25.0	40.0
66-67	33.55743285089899	35.5	33.0	37.5	24.5	40.0
68-69	33.41678838790145	35.5	32.5	37.0	23.0	40.0
70-71	32.99668678284342	35.0	32.0	37.0	23.5	39.0
72-73	32.76710223564909	35.0	32.0	36.5	23.0	39.0
74-75	32.56349451476929	35.0	31.5	37.0	22.0	38.5
76-77	31.02117453484525	33.5	29.5	36.0	21.0	36.5
78-79	32.1494593995336	35.0	31.5	36.5	23.0	37.0
80-81	32.2709765367574	35.0	32.0	36.0	23.0	37.5
82-83	32.184446077224884	35.0	31.5	37.0	23.0	37.0
84-85	31.86186556827998	35.0	31.5	35.5	22.0	36.5
86-87	31.740998661066417	35.0	31.0	36.0	21.5	36.0
88-89	31.68891142340383	35.0	31.0	35.5	22.0	37.0
90-91	31.453970404581007	34.5	31.0	35.0	21.5	36.5
92-93	31.235960501191208	34.0	30.5	35.0	21.0	36.5
94-95	31.35196841199903	34.0	31.0	35.0	20.0	36.5
96-97	31.089518528170423	34.0	30.5	35.0	20.0	37.0
98-99	30.934163490922117	34.0	30.0	35.0	20.0	36.0
100	29.691520319671064	33.0	29.0	35.0	18.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.08532925226405297
1101	2	-0.1981900839143762
1101	3	-0.15679820011082768
1101	4	-0.1354406772115695
1101	5	-0.23955863469248584
1101	6	-0.14113972851014722
1101	7	-0.13188605842007917
1101	8	-0.26693556520293527
1101	9	-0.1507662679026467
1101	10-11	-0.16958135048712109
1101	12-13	-0.17600835602969056
1101	14-15	-0.34892899388278664
1101	16-17	-0.3729469214662231
1101	18-19	-0.47598006462591513
1101	20-21	-0.3603873669357682
1101	22-23	-0.8363982390473623
1101	24-25	-0.3673936052775133
1101	26-27	-0.39034811207926623
1101	28-29	-0.4325120188332363
1101	30-31	-0.5043928011411296
1101	32-33	-0.42326113071840155
1101	34-35	-0.4789920503912981
1101	36-37	-0.5146236197516671
1101	38-39	-0.42316489529277135
1101	40-41	-0.4288179694298222
1101	42-43	-0.43759185556699265
1101	44-45	-0.4661612242962576
1101	46-47	-0.28938411708210765
1101	48-49	-0.44297673577637653
1101	50-51	-0.4985298140382284
1101	52-53	-0.4714918849706251
1101	54-55	-0.4339011813226463
1101	56-57	-0.6510995418987235
1101	58-59	-0.5374827523038519
1101	60-61	-0.7176124389183016
1101	62-63	-0.5472907718952413
1101	64-65	-0.5448958876624275
1101	66-67	-0.6102757902638203
1101	68-69	-0.5713699716031684
1101	70-71	-0.6591032658761122
1101	72-73	-0.5721486982405395
1101	74-75	-0.5651802883686301
1101	76-77	-0.698659833503612
1101	78-79	-0.6520460489242836
1101	80-81	-0.5443104494382709
1101	82-83	-0.4731667268845996
1101	84-85	-0.4951830349460842
1101	86-87	-0.7243517053073063
1101	88-89	-0.6822796661418415
1101	90-91	-0.7451819495998855
1101	92-93	-0.6294575129855886
1101	94-95	-0.737590072944073
1101	96-97	-0.7747639271863065
1101	98-99	-0.80732145970833
1101	100	-0.8325247157926441
1102	1	-0.05598397071251782
1102	2	-0.13714241588620268
1102	3	-0.17291252299596493
1102	4	-0.14200182624961855
1102	5	-0.11278075237338214
1102	6	-0.2015421612664241
1102	7	-0.13372966255768404
1102	8	-0.1896910317962437
1102	9	-0.1578472194219529
1102	10-11	-0.20509894718167487
1102	12-13	-0.17864461629360306
1102	14-15	-0.3299377829811192
1102	16-17	-0.3057896353005347
1102	18-19	-0.28482224648092824
1102	20-21	-0.30120631876981463
1102	22-23	-0.2877621155980705
1102	24-25	-0.1922388318007151
1102	26-27	-0.25831522794101147
1102	28-29	-0.26847948278115297
1102	30-31	-0.35222412461548913
1102	32-33	-0.2584208047800658
1102	34-35	-0.25587189605447236
1102	36-37	-0.23167481641809928
1102	38-39	-0.3794820582601588
1102	40-41	-0.2636920992046967
1102	42-43	-0.32006355087928284
1102	44-45	-0.2573845490326576
1102	46-47	-0.2889593926173646
1102	48-49	-0.2954307782024088
1102	50-51	-0.29796337320617283
1102	52-53	-0.37393551893753596
1102	54-55	-0.3207343467530066
1102	56-57	-0.3304068116334804
1102	58-59	-0.34826602065154333
1102	60-61	-0.45946956474563905
1102	62-63	-0.6314318207871494
1102	64-65	-0.5714586708750886
1102	66-67	-0.6260603503617759
1102	68-69	-0.5263483445779613
1102	70-71	-0.5791421207625689
1102	72-73	-0.5967482226933996
1102	74-75	-0.614687625828175
1102	76-77	-0.47220737258943046
1102	78-79	-0.5178360459297195
1102	80-81	-0.5096700291257648
1102	82-83	-0.5022505463144284
1102	84-85	-0.5486543898505722
1102	86-87	-0.4867861880514859
1102	88-89	-0.545246669154789
1102	90-91	-0.5886853376619108
1102	92-93	-0.5059430064089838
1102	94-95	-0.6776770975898607
1102	96-97	-0.6843461683230068
1102	98-99	-0.7754859814594148
1102	100	-0.8950131643032329
1103	1	-0.07195535609036341
1103	2	-0.12740808888688804
1103	3	-0.2186698685027082
1103	4	-0.12624320476567874
1103	5	-0.18458523265968552
1103	6	-0.19564152442953997
1103	7	-0.12692349107119583
1103	8	-0.08957213254327456
1103	9	-0.16781469261128024
1103	10-11	-0.22610676761864568
1103	12-13	-0.20416090212703608
1103	14-15	-0.24247477419657315
1103	16-17	-0.3252216542056061
1103	18-19	-0.28914098225121165
1103	20-21	-0.28688237604269773
1103	22-23	-0.3266674862800585
1103	24-25	-0.2619434809645682
1103	26-27	-0.253832562439797
1103	28-29	-0.26182977761977355
1103	30-31	-0.4407549687122909
1103	32-33	-0.32669198751697337
1103	34-35	-0.44360894443128984
1103	36-37	-0.43168791715786625
1103	38-39	-0.34477059125613607
1103	40-41	-0.4061703348525256
1103	42-43	-0.4019768923271485
1103	44-45	-0.2901768978369361
1103	46-47	-0.40841737811305023
1103	48-49	-0.36929690121994696
1103	50-51	-0.3776636443437411
1103	52-53	-0.3073936490035649
1103	54-55	-1.065942953870838
1103	56-57	-0.346128646482633
1103	58-59	-0.3775790963566905
1103	60-61	-0.3300874089685166
1103	62-63	-0.3611378353375372
1103	64-65	-0.37409151798332374
1103	66-67	-0.3869416171980049
1103	68-69	-0.36576513661974985
1103	70-71	-0.41020323219023425
1103	72-73	-0.4714537401885295
1103	74-75	-0.5481315110181058
1103	76-77	-0.41883833816168803
1103	78-79	-0.39872861319584274
1103	80-81	-1.227357573527346
1103	82-83	-0.17864602135377083
1103	84-85	-0.17971448595196193
1103	86-87	-0.24673824192015203
1103	88-89	-0.3230657377096193
1103	90-91	-0.1740230185112246
1103	92-93	-0.19189398979036554
1103	94-95	-0.2540653592664164
1103	96-97	-0.47953572670759925
1103	98-99	-0.39735229814664663
1103	100	-0.24970444146851278
1104	1	-0.11638795707666816
1104	2	-0.22870740104458065
1104	3	-0.17976316582603147
1104	4	-0.045508083480754635
1104	5	-0.08589334571173168
1104	6	-0.2884333053745962
1104	7	-0.2111996337244193
1104	8	-0.1245232970064265
1104	9	-0.2216295598029916
1104	10-11	-0.2307834399544859
1104	12-13	-0.12645303963275722
1104	14-15	-0.3237188315189812
1104	16-17	-0.31490207766814393
1104	18-19	-0.3050136440941458
1104	20-21	-0.33317487297483694
1104	22-23	-0.27711975194851135
1104	24-25	-0.18556042833477449
1104	26-27	-0.22772637222102787
1104	28-29	-0.40296260129057515
1104	30-31	-0.35512618817386965
1104	32-33	-0.25033500951634835
1104	34-35	-0.326730069624503
1104	36-37	-0.4089968623356057
1104	38-39	-0.3152495672996096
1104	40-41	-0.22395083375992186
1104	42-43	-0.23330356644277117
1104	44-45	-0.23418294202270573
1104	46-47	-0.2837050851565479
1104	48-49	-0.4004119843389944
1104	50-51	-0.2959260544343536
1104	52-53	-0.32498459685762526
1104	54-55	-0.35357648689221577
1104	56-57	-0.29999621723066383
1104	58-59	-0.38273844156411485
1104	60-61	-0.37021524516419646
1104	62-63	-0.3558797534468354
1104	64-65	-0.7290115271296145
1104	66-67	-0.5748355081254388
1104	68-69	-0.42319359296549663
1104	70-71	-0.3175918997453877
1104	72-73	-0.34883803218335174
1104	74-75	-0.39210275886577506
1104	76-77	-0.32461937191006385
1104	78-79	-0.32694070962166677
1104	80-81	-0.30784574940888376
1104	82-83	-0.27535139109087936
1104	84-85	-0.9174448740600916
1104	86-87	-0.42915862889404366
1104	88-89	-0.37771372983873874
1104	90-91	-0.4380322587715675
1104	92-93	-0.34604103229770544
1104	94-95	-0.33118734388543913
1104	96-97	-0.27860570897609804
1104	98-99	-0.3147726278053753
1104	100	-1.6164096928045595
1105	1	-0.03380420339152579
1105	2	-0.15368279824655673
1105	3	-0.18760710430146332
1105	4	-0.08646405652165612
1105	5	-0.0836939659806788
1105	6	-0.11694393983203355
1105	7	-0.10720573310608472
1105	8	-0.21801608015366725
1105	9	-0.14951664174504486
1105	10-11	-0.08074118554897325
1105	12-13	-0.1869177785665812
1105	14-15	-0.2741478755132931
1105	16-17	-0.22511636251833522
1105	18-19	-0.34268183842255695
1105	20-21	-0.28303195112061985
1105	22-23	-0.23328500671060226
1105	24-25	-0.20794232098266008
1105	26-27	-0.2947912089707785
1105	28-29	-0.26905791017787095
1105	30-31	-0.32327000523389415
1105	32-33	-0.19162697276873786
1105	34-35	-0.2515026725630918
1105	36-37	-0.3759452954470035
1105	38-39	-0.3517329388804882
1105	40-41	-0.28122194228854624
1105	42-43	-0.33715556292262505
1105	44-45	-0.37740212285370234
1105	46-47	-0.36595843646568227
1105	48-49	-0.33568877709808476
1105	50-51	-0.24428318279385053
1105	52-53	-0.39868620759440887
1105	54-55	-1.2761245168345638
1105	56-57	-0.3376156860176067
1105	58-59	-0.2983453141025194
1105	60-61	-0.3209162592891275
1105	62-63	-0.26026112533148904
1105	64-65	-0.27773628129845207
1105	66-67	-0.31453841385750536
1105	68-69	-0.19680865945978354
1105	70-71	-0.15811201201671565
1105	72-73	-0.27887169745203977
1105	74-75	-0.3743700989658336
1105	76-77	-0.3645679887863622
1105	78-79	-0.1663227564630887
1105	80-81	-0.12547241325410852
1105	82-83	-0.10285621515951249
1105	84-85	-0.11955636539577696
1105	86-87	-0.15011437534917604
1105	88-89	-0.2878045350458329
1105	90-91	-0.32644376003478115
1105	92-93	-0.21877804053217886
1105	94-95	-0.2880847971190015
1105	96-97	-0.2707756536795678
1105	98-99	-0.11941677197460265
1105	100	-0.10859607457738463
1106	1	-0.019295145198952923
1106	2	-0.12813755105712232
1106	3	-0.07328893969036443
1106	4	-0.10719189442687593
1106	5	-0.12666151670198644
1106	6	-0.15496403842187334
1106	7	-0.12551267367808805
1106	8	-0.15190375822754731
1106	9	-0.17412895080214952
1106	10-11	-0.23589998079086172
1106	12-13	-0.18764963123003042
1106	14-15	-0.21121540367155234
1106	16-17	-0.214431225333648
1106	18-19	-0.20947674439511843
1106	20-21	-0.2043532292828658
1106	22-23	-0.12324742541738942
1106	24-25	-0.07612785128299748
1106	26-27	-0.08698110459269515
1106	28-29	-0.10485588611599184
1106	30-31	-0.16785790636451026
1106	32-33	-0.04668692079293635
1106	34-35	-0.026330777223343205
1106	36-37	-0.15138941281171725
1106	38-39	-0.1562919063229984
1106	40-41	-0.14473080528534865
1106	42-43	-0.18771482043470655
1106	44-45	-0.14617386045872394
1106	46-47	-0.23020364219245693
1106	48-49	-0.18932368321342352
1106	50-51	-0.12017388330246348
1106	52-53	-0.2201438973593639
1106	54-55	-0.23313918432258873
1106	56-57	-0.1585531104590885
1106	58-59	-0.2239128895097977
1106	60-61	-0.19952484027593442
1106	62-63	-0.2172837302802222
1106	64-65	-0.12118717171246374
1106	66-67	-0.1055918247957166
1106	68-69	0.04883896312514224
1106	70-71	-0.07983985724882103
1106	72-73	-0.016450097489162374
1106	74-75	-0.09232292546270315
1106	76-77	-0.11873768249055772
1106	78-79	-0.0063508823149618365
1106	80-81	0.038637949575203834
1106	82-83	-0.02866292887981814
1106	84-85	-0.03988919016802228
1106	86-87	0.08840505996953496
1106	88-89	-0.039493673650053296
1106	90-91	0.008267873426998307
1106	92-93	0.1355295489389725
1106	94-95	0.03104867264472233
1106	96-97	0.061942146014995814
1106	98-99	-0.054015984691222485
1106	100	0.0623091397425064
1107	1	-0.059787562504411085
1107	2	-0.09332670418481825
1107	3	-0.08562618864169735
1107	4	-0.05130952866105787
1107	5	-0.11157025402419407
1107	6	-0.13226904238981518
1107	7	-0.18607677557135816
1107	8	-0.10000317916956192
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2308	84-85	0.4331133081701317
2308	86-87	0.4594813152841901
2308	88-89	0.3617865921799428
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2308	92-93	0.44416714320328765
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2309	4	0.18062216791312125
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2310	7	0.12401888304605535
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2310	10-11	0.23210645667666086
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2312	4	-0.02684903587691423
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2312	26-27	-2.588692069932563
2312	28-29	0.010559730227925002
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2312	64-65	0.2298798177338952
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2312	72-73	0.3329953987167684
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2312	84-85	0.18688401500213203
2312	86-87	0.21595511071951634
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2312	90-91	0.26974871942201517
2312	92-93	0.40964343508902346
2312	94-95	0.31700056073883687
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2313	64-65	-0.5350368621843771
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2313	96-97	0.05625859765073571
2313	98-99	0.28271088872112315
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2314	1	-1.887016827580382
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2314	4	-0.05437956243984132
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2314	10-11	-0.7836376570765395
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2314	66-67	0.46741535740452633
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2314	76-77	0.6298399359910469
2314	78-79	0.49938602997166015
2314	80-81	0.3355705339323052
2314	82-83	0.3644052852740529
2314	84-85	0.35105796227754027
2314	86-87	0.278163023171043
2314	88-89	0.21517917200284842
2314	90-91	0.2553498909935641
2314	92-93	0.12241304178840551
2314	94-95	-0.03464621292628678
2314	96-97	0.109473545936698
2314	98-99	0.16527304617020988
2314	100	0.15016866000658524
2315	1	0.1037392187236037
2315	2	0.038746956753776374
2315	3	0.027049924311775442
2315	4	-0.11407357247575334
2315	5	-0.027691246889233412
2315	6	-0.037564741314547234
2315	7	0.03710632160480287
2315	8	0.06279427837239382
2315	9	0.0719968270665845
2315	10-11	0.11231729440097382
2315	12-13	0.03545514828855545
2315	14-15	0.07855950391903121
2315	16-17	0.19829224375246213
2315	18-19	0.13513076508584732
2315	20-21	0.17963526975576372
2315	22-23	0.19068084011534836
2315	24-25	0.2975292876760065
2315	26-27	0.13661845376230275
2315	28-29	-1.1551139503061094
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2315	32-33	-1.4301689682268233
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2315	62-63	0.2702011418625361
2315	64-65	0.41306110958608855
2315	66-67	0.3025630693029626
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2315	80-81	0.20703034155788202
2315	82-83	0.1843156483057271
2315	84-85	0.10817780444785186
2315	86-87	0.18963636016939134
2315	88-89	0.18039343905785188
2315	90-91	0.2633269573420769
2315	92-93	0.2958882970724659
2315	94-95	0.1824512492441066
2315	96-97	0.17471483274770705
2315	98-99	0.22677574229738084
2315	100	0.1276319221060156
2316	1	0.010334362410112874
2316	2	-0.1387832350677911
2316	3	-0.12601294029450116
2316	4	-0.045751456595397144
2316	5	-0.11181561375931182
2316	6	-0.106760662411169
2316	7	-0.035131466525946564
2316	8	-0.01577185616829979
2316	9	-0.20343574791693442
2316	10-11	-0.027596457758932047
2316	12-13	-0.008277810713060774
2316	14-15	0.01604081076792596
2316	16-17	-1.3330439064426685
2316	18-19	0.07581407670294737
2316	20-21	-0.093280486859868
2316	22-23	-0.026593615650696734
2316	24-25	0.11377168308653296
2316	26-27	-0.047261292954488
2316	28-29	0.05560245904656114
2316	30-31	0.06319136811477932
2316	32-33	0.10652804649335224
2316	34-35	0.09365548258337952
2316	36-37	0.06737018783396564
2316	38-39	0.03491260407220409
2316	40-41	0.05188957084710211
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2316	48-49	0.022455731131259427
2316	50-51	0.020676367504023574
2316	52-53	-0.006086274368733768
2316	54-55	0.1092976286846934
2316	56-57	0.1352120048381451
2316	58-59	0.030090418738815572
2316	60-61	-1.284261681239478
2316	62-63	0.10809265909335863
2316	64-65	0.12078804428426793
2316	66-67	-0.027459787429222615
2316	68-69	0.19876640834265658
2316	70-71	0.09701718725587227
2316	72-73	0.2097215405663988
2316	74-75	0.09940861208044538
2316	76-77	-0.19862639228037438
2316	78-79	-0.28552977815359526
2316	80-81	-0.062258701584006815
2316	82-83	-0.15613286233523382
2316	84-85	-0.7035376612639261
2316	86-87	-0.060744877000065145
2316	88-89	0.00538255750353045
2316	90-91	0.138435851001244
2316	92-93	0.24128771963923157
2316	94-95	0.16620849450012187
2316	96-97	0.19739674921361328
2316	98-99	0.2651196290979705
2316	100	0.11618457383065817
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	7.0
12	69.0
13	282.0
14	1173.0
15	3131.0
16	6954.0
17	12693.0
18	20391.0
19	29183.0
20	38517.0
21	50098.0
22	62436.0
23	72771.0
24	80077.0
25	89297.0
26	102129.0
27	119410.0
28	143979.0
29	173621.0
30	212647.0
31	261907.0
32	325744.0
33	421110.0
34	599374.0
35	1060559.0
36	1407473.0
37	1093476.0
38	918178.0
39	152911.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.618439004645886	17.732633735236107	12.739125577482929	23.90980168263508
2	17.089650608560607	20.163235603262244	38.58274504378119	24.164368744395965
3	19.5464463029722	25.555882647860468	29.426390021647077	25.47128102752026
4	13.636732286910934	16.175206333711323	35.64083999239106	34.547221386986685
5	14.095369826455292	35.971617374257235	33.391986791845284	16.541026007442188
6	31.579202071513656	34.446837164023165	18.61670482297733	15.357255941485853
7	28.451936466877616	31.64616709483586	21.40774897756844	18.49414746071809
8	27.27237751293121	33.579081634362936	20.131627894818333	19.01691295788752
9	27.601643933751475	15.612021875504379	19.872224876495846	36.9141093142483
10-11	25.88502101467659	25.03046549997034	27.66699722206117	21.417516263291898
12-13	26.166261151662614	22.99945037635814	27.776712044130758	23.057576427848492
14-15	23.766241061651144	24.23717017801083	25.88102463750551	26.115564122832513
16-17	23.161514114346357	26.827342460284665	26.5531103026179	23.458033122751083
18-19	22.93305088994601	25.780671681547133	28.63962005805622	22.646657370450633
20-21	23.710578509183797	25.15309456733969	28.53525086113809	22.60107606233842
22-23	23.511850541388384	24.89387859993348	28.40582834212534	23.188442516552794
24-25	23.169181187611773	25.098607919735528	28.38990446875997	23.34230642389273
26-27	23.120525869636698	25.04998825166312	28.483888019076215	23.345597859623965
28-29	23.09556728906086	25.348366789241688	28.533829130354192	23.02223679134326
30-31	23.731051917070186	25.131057601528582	28.42682345761472	22.71106702378651
32-33	23.39053545689517	25.28077486737905	28.337148230803454	22.99154144492233
34-35	23.53708192561085	25.389520813400768	28.196973201986943	22.876424059001437
36-37	23.65379537814425	25.343054383357423	28.05100467853685	22.95214555996148
38-39	23.593980802525255	25.522924768477324	27.863264259773267	23.019830169224157
40-41	23.495528148496557	25.354952605620078	28.07644527705491	23.073073968828453
42-43	23.376481217103336	25.49865246094295	27.806521732427257	23.31834458952645
44-45	23.24655606849856	25.598316694898273	27.806233564615734	23.348893671987437
46-47	23.303861207767223	25.501158197419265	27.602324197939744	23.59265639687377
48-49	23.409684853473316	25.637650175080655	27.483765159629332	23.468899811816698
50-51	23.24952203486479	25.953759910833917	27.329630459661043	23.467087594640248
52-53	23.416593515285037	26.025059501484648	26.939383061525255	23.618963921705063
54-55	23.214216171089	25.83160760075301	27.262937296837507	23.69123893132048
56-57	23.363409748667735	25.957087501207372	26.926590564360602	23.752912185764288
58-59	23.290754416113614	25.87283249858492	27.23332181247347	23.603091272827996
60-61	23.570950320709503	25.91759622809992	27.25021655340023	23.261236897790347
62-63	23.36789121325787	26.02703421161745	27.149746389211625	23.455328185913054
64-65	23.292455148322784	25.75237179580846	27.1530487450941	23.80212431077466
66-67	23.22116394541217	25.839417095609946	27.0113749899291	23.928043969048787
68-69	23.066553971628736	25.854482643981093	27.035628969684943	24.043334414705228
70-71	23.014003062340425	25.771282421409893	27.112597983767074	24.102116532482608
72-73	22.994582903983606	26.055308847266605	26.752418864714972	24.19768938403482
74-75	23.0239480558933	26.113528728798883	26.677231627287924	24.18529158801989
76-77	22.89358938179566	25.801771354148162	26.930501865800448	24.374137398255726
78-79	22.83159145222686	25.920030072057244	26.897854543984774	24.35052393173112
80-81	22.83523761928151	26.075396961578967	26.593825137432464	24.495540281707058
82-83	22.84484174291849	26.06643409843661	26.649112560011574	24.43961159863333
84-85	22.788253547620478	26.214976899714305	26.441944713745208	24.554824838920013
86-87	22.639454348747424	26.10917435454127	26.62251706232603	24.62885423438528
88-89	22.443756253224432	25.914528312750267	26.718372835513833	24.92334259851147
90-91	22.843317966172584	25.822125226050087	26.673568260087965	24.660988547689364
92-93	22.647846692076783	25.883229423989334	26.61426458626788	24.854659297666004
94-95	22.429575102318584	25.720828136217616	26.664188003578982	25.18540875788482
96-97	22.291002414098937	25.62515483586594	26.828886393640765	25.25495635639436
98-99	22.33591344342816	25.568627398409845	26.64236698066794	25.453092177494057
100	22.164240729470244	25.682309917610187	26.829305893298084	25.324143459621485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3430.0
1	2591.5
2	5287.5
3	7335.0
4	4173.0
5	2730.5
6	3730.5
7	6922.0
8	10521.5
9	11782.0
10	10096.0
11	8003.5
12	7921.5
13	8557.5
14	9335.0
15	10074.0
16	10644.0
17	11357.5
18	12157.5
19	12810.5
20	13707.0
21	14915.5
22	16361.0
23	18448.5
24	21312.0
25	25133.5
26	30773.0
27	37134.5
28	43393.5
29	51140.0
30	60686.0
31	70383.5
32	80941.5
33	91818.5
34	102852.0
35	112978.0
36	126091.0
37	140971.0
38	151339.0
39	160909.0
40	169428.5
41	178058.5
42	189197.0
43	202889.0
44	216654.5
45	230650.5
46	246203.5
47	264045.0
48	285331.5
49	303663.0
50	321761.0
51	326216.5
52	313387.5
53	305456.0
54	301313.5
55	289286.5
56	267227.0
57	244874.0
58	225835.0
59	201786.0
60	172257.0
61	143387.5
62	118624.0
63	94645.0
64	71228.0
65	54028.5
66	40988.5
67	32305.5
68	26010.5
69	20117.0
70	15915.0
71	12706.0
72	10594.5
73	8954.5
74	7153.5
75	5546.0
76	4145.0
77	2807.5
78	1857.5
79	1278.0
80	938.5
81	654.5
82	444.5
83	282.5
84	164.0
85	112.5
86	82.5
87	51.5
88	33.0
89	22.0
90	12.5
91	7.5
92	4.0
93	5.0
94	4.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.045404165440070525
2	0.010134499283346123
3	0.0
4	3.8875724764158404E-4
5	4.5578435930392617E-4
6	2.681084466493683E-5
7	0.0
8	4.0216266997405246E-5
9	0.0
10-11	6.7027111662342074E-6
12-13	2.4129760198443148E-4
14-15	0.010670716176644859
16-17	0.0015081100124026967
18-19	2.681084466493683E-5
20-21	8.445416069455102E-4
22-23	6.836765389558892E-4
24-25	0.00439697852504964
26-27	0.01562401972849194
28-29	0.013009962373660595
30-31	0.009839579992031817
32-33	0.0071517928143719
34-35	0.008894497717592794
36-37	0.005248222843161385
38-39	0.004041734833239227
40-41	0.005328655377156195
42-43	0.006903792501221234
44-45	0.007145090103205665
46-47	0.014678937454052916
48-49	0.016736669782086815
50-51	0.01644175049077251
52-53	0.010999149023790334
54-55	0.003693193852595049
56-57	0.003746815541924922
58-59	0.009839579992031817
60-61	0.0025671383766677014
62-63	1.2064880099221574E-4
64-65	3.3513555831171036E-4
66-67	7.641090729506996E-4
68-69	1.0724337865974732E-4
70-71	1.876759126545578E-4
72-73	0.0030631390029690325
74-75	0.0056235746684705
76-77	0.005402385199984772
78-79	0.003371463716615807
80-81	5.362168932987366E-5
82-83	8.713524516104471E-4
84-85	2.6810844664936834E-4
86-87	4.222708034727551E-4
88-89	2.2118946848572886E-4
90-91	5.094060486337998E-4
92-93	5.429196044649708E-4
94-95	2.0108133498702623E-5
96-97	0.002091245883865073
98-99	5.094060486337998E-4
100	1.0724337865974732E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7459668.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.58466324646022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.82379434794578	36.01836918028876
2	17.11398549303118	17.656383569256377
3	5.944135082921485	9.198786196319238
4	2.5591631207723116	5.2805427111119965
5	1.3286185155984902	3.426816935507998
6	0.7474075567151118	2.31328602726052
7	0.4937386161995729	1.7828538173899764
8	0.33289588232852274	1.3737857588840072
9	0.2535804765220398	1.17727771385397
>10	1.2754307213203877	11.804967646198023
>50	0.08427096607321982	2.985471332431302
>100	0.040021137089562765	3.666144655595674
>500	0.0018176210908916934	0.6259209331669088
>1k	8.553467936262037E-4	0.7542788609632941
>5k	1.4255779892571651E-4	0.43310181840831313
>10k+	1.4255779892571651E-4	1.5020128433635787
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28190	0.37789885555228464	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25359	0.3399481049290666	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16011	0.2146342169651518	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12140	0.16274182711616655	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.702711166234207E-5
7	0.0	0.0	0.0	0.0	6.702711166234207E-5
8	0.0	0.0	0.0	0.0	8.043253399481049E-5
9	0.0	0.0	0.0	0.0	1.0724337865974732E-4
10-11	0.0	0.0	0.0	6.7027111662342074E-6	1.0724337865974732E-4
12-13	0.0	0.0	0.0	1.3405422332468415E-5	1.2064880099221574E-4
14-15	0.0	0.0	0.0	1.3405422332468415E-5	1.4745964565715257E-4
16-17	0.0	0.0	0.0	2.0108133498702623E-5	1.6086506798962099E-4
18-19	0.0	0.0	0.0	4.0216266997405246E-5	1.6086506798962099E-4
20-21	0.0	0.0	0.0	8.043253399481049E-5	1.6086506798962099E-4
22-23	0.0	0.0	0.0	2.547030243168999E-4	1.809732014883236E-4
24-25	0.0	0.0	0.0	6.300548496260155E-4	2.0108133498702624E-4
26-27	0.0	0.0	0.0	0.001575137124065039	2.0108133498702624E-4
28-29	0.0	0.0	0.0	0.004450600214379514	2.0108133498702624E-4
30-31	0.0	0.0	0.0	0.012936232550832021	2.2789217965196306E-4
32-33	0.0	0.0	0.0	0.03392242121231133	2.8151386898183674E-4
34-35	0.0	0.0	0.0	0.06567316400676276	3.083247136467736E-4
36-37	0.0	0.0	0.0	0.11694220171728822	3.2173013597924197E-4
38-39	0.0	0.0	0.0	0.19474057022376867	3.284328471454762E-4
40-41	0.0	0.0	0.0	0.2902877178984373	3.485409806441788E-4
42-43	0.0	0.0	0.0	0.3792997221860276	3.485409806441788E-4
44-45	0.0	0.0	0.0	0.48664364151326844	3.485409806441788E-4
46-47	0.0	0.0	0.0	0.61753418516749	3.7535182530911565E-4
48-49	0.0	0.0	0.0	0.7563808469760316	4.021626699740525E-4
50-51	0.0	0.0	0.0	0.902660815467927	4.021626699740525E-4
52-53	0.0	0.0	0.0	1.0490681354719809	4.2897351463898933E-4
54-55	0.0	0.0	0.0	1.2209524606188908	4.490816481376919E-4
56-57	0.0	0.0	0.0	1.4177105469036961	4.8259520396886296E-4
58-59	0.0	1.3405422332468415E-5	0.0	1.64140012665443	4.960006263013313E-4
60-61	0.0	1.3405422332468415E-5	0.0	1.8666715462403958	4.960006263013313E-4
62-63	0.0	1.3405422332468415E-5	0.0	2.101869681063554	5.094060486337998E-4
64-65	0.0	1.3405422332468415E-5	0.0	2.33974219764204	5.228114709662681E-4
66-67	0.0	4.0216266997405246E-5	0.0	2.6115706489886685	5.429196044649708E-4
68-69	0.0	4.0216266997405246E-5	0.0	2.926490830423016	5.49622315631205E-4
70-71	0.0	4.0216266997405246E-5	0.0	3.263389201771446	5.898385826286103E-4
72-73	0.0	4.0216266997405246E-5	0.0	3.5796231145943764	6.970819612883576E-4
74-75	0.0	4.0216266997405246E-5	0.0	3.9044297949989195	6.970819612883576E-4
76-77	0.0	4.0216266997405246E-5	0.0	4.267059874514523	6.970819612883576E-4
78-79	0.0	4.0216266997405246E-5	0.0	4.672285683491545	6.970819612883576E-4
80-81	0.0	4.0216266997405246E-5	0.0	5.08793420833206	7.037846724545918E-4
82-83	0.0	4.0216266997405246E-5	0.0	5.512571068846496	7.372982282857629E-4
84-85	0.0	4.0216266997405246E-5	0.0	5.937569875763908	7.372982282857629E-4
86-87	0.0	4.0216266997405246E-5	0.0	6.382442221289205	7.440009394519971E-4
88	0.0	4.0216266997405246E-5	0.0	6.738718130619218	7.507036506182313E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	12705	0.0	53.379356	2
GTACCTG	15070	0.0	45.801617	1
ACCTGGG	15195	0.0	44.101852	3
CCTGGGG	13725	0.0	34.033924	4
TATAACG	705	0.0	33.332092	2
GGTATCA	25795	0.0	29.27373	1
GTATCAA	36450	0.0	27.050102	1
TATCACG	795	0.0	25.420435	2
CTGGGGG	8300	0.0	24.968681	5
TAACGCA	1005	0.0	24.782652	4
ATAACGC	985	0.0	23.37749	3
TCAACGC	43055	0.0	22.637222	4
CAACGCA	43610	0.0	22.316654	5
TATCAAC	44000	0.0	22.20667	2
ATCAACG	43945	0.0	22.168066	3
AACGCAG	44750	0.0	21.905659	6
GTACACG	1940	0.0	21.81267	1
GTACATG	35285	0.0	20.814161	1
TGGGGGG	11425	0.0	20.48369	6
GAGTACT	27315	0.0	19.90003	12-13
>>END_MODULE