FastQCFastQC Report
Mon 6 Jun 2016
ERR523152_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523152_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8414746
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303710.36092592693825815No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT279530.3321906567352122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228860.27197493542882933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129680.1541104152163357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG117750.047.4882552
ACCTGGG129950.042.089013
TATAACG9950.037.812222
GTACCTG162400.035.0396231
ATAACGC12450.031.352633
TAACGCA12550.030.3531654
CCTGGGG121300.028.8452844
GTACATG322350.024.9329991
TATCACG7100.024.5080432
TACATGG326600.024.0472582
ACATGGG324550.023.1848163
CATGGGG194500.022.6317924
TACCGGG9200.022.4921072
CCGTATC24850.021.74500794
GAGTACT265650.020.29318412-13
GTATAAC25150.020.1950931
ATGGGGG116100.019.8475045
GTATCAA617400.018.6011241
GAGTACG10750.018.3738841
AGTACTT277400.018.08664512-13
Produced by FastQC (version 0.11.5)