FastQCFastQC Report
Mon 6 Jun 2016
ERR523152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8414746
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT285310.3390595509359403No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284440.3380256516358307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232480.27627690722928533No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT152460.18118193941920527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128270.1524347853161581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG131900.047.43052
ACCTGGG138650.043.628243
TATAACG8550.037.9321482
GTACCTG171650.036.6595571
TAACGCA10400.033.4431654
ATAACGC10250.033.015483
CCTGGGG127200.031.2971884
TATCACG8450.023.3623772
GTACATG312050.023.0590881
CTGGGGG75650.022.5532555
TACATGG315900.022.110252
CATGGGG179050.021.3677644
ACATGGG312950.021.2515413
GAGTACT269350.020.26693212-13
ATGGGGG106300.019.1896925
GTATAAC25700.019.0315911
TACCGGG9750.018.319072
AGTACTT282150.018.18148812-13
GTACTTT302850.017.79932414-15
AGAGTAC447900.017.62844310-11
Produced by FastQC (version 0.11.5)