FastQCFastQC Report
Mon 6 Jun 2016
ERR523151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10275940
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379490.36929954826517086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297290.2893068663304768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164900.16047193736047505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187200.051.89791
GTATCAA344700.042.748021
TCAACGC424250.034.2498974
ATCAACG431100.033.696923
CAACGCA431400.033.6815875
TATCAAC439450.033.2431532
AACGCAG447000.032.582126
ACGCAGA508750.028.6086867
CGCAGAG523900.027.7615578
GTACATG438400.027.22611
TACATGG452100.025.8462332
ACATGGG454900.024.9913183
GCAGAGT579250.024.6780159
CATGGGG298850.023.3672284
GAGTACT315500.021.88874612-13
CAGAGTA564900.020.85494610-11
GTGGTAT96850.020.5968951
AGAGTAC523250.020.42327110-11
GTACTTT341000.020.08829714-15
ATGGGGG166800.019.9184955
Produced by FastQC (version 0.11.5)