FastQCFastQC Report
Mon 6 Jun 2016
ERR523151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10275940
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340300.331161918033776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285350.27768749136332055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155870.15168442011144478No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA194600.052.6557041
GTATCAA349350.040.979961
TCAACGC431900.032.772494
ATCAACG436800.032.3531073
CAACGCA438850.032.253485
TATCAAC448650.031.732012
AACGCAG455850.031.1021966
ACGCAGA524350.026.9404857
CGCAGAG538800.026.1726448
GTACATG442150.024.3347511
TACATGG452150.023.414552
GCAGAGT595700.023.3570549
ACATGGG458050.022.4491353
GAGTACT323500.021.28028912-13
CATGGGG296850.020.8807184
CAGAGTA584200.020.28891210-11
AGAGTAC544900.019.96217510-11
GTACTTT345850.019.75962814-15
GTGGTAT103000.019.1384661
AGTACTT344300.018.29490712-13
Produced by FastQC (version 0.11.5)