FastQCFastQC Report
Mon 6 Jun 2016
ERR523150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7574030
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267280.35289007305225883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220050.2905322529749684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113660.15006542091858627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG105150.044.1457252
GGTATCA164250.042.14861
ACCTGGG116550.039.0607873
GTATCAA267750.036.3071171
GTACCTG135250.034.9817471
TATAACG7700.031.7609422
TCAACGC314750.030.2279034
CAACGCA319100.029.7688645
ATCAACG319250.029.74293
TATCAAC324700.029.3742282
AACGCAG330200.028.9129646
CCTGGGG114000.027.1043154
TAACGCA8850.027.1022174
ACGCAGA374850.025.3666847
GTACATG289000.025.2077661
CGCAGAG386050.024.55648
TACATGG295950.024.2661842
ATAACGC10000.023.5151583
ATGGGGG128200.023.256215
TATCACG7400.022.8797662
Produced by FastQC (version 0.11.5)