FastQCFastQC Report
Mon 6 Jun 2016
ERR523150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7574030
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253790.3350792114633821No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223500.2950872917060006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115050.15190063942181375No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167050.043.2738721
TACCTGG106850.042.6689152
TATAACG7050.039.334832
ACCTGGG118600.037.3312033
GTATCAA263450.033.8305741
GTACCTG139200.033.1795581
TAACGCA8350.031.5215344
ATAACGC9450.029.8418123
TCAACGC311500.028.0043644
ATCAACG318500.027.4183983
CAACGCA318400.027.3978445
TATCAAC321650.027.3549982
CCTGGGG107750.026.9573364
AACGCAG332550.026.4698936
GTACATG286300.025.1179681
TACATGG292250.024.4296722
ACATGGG294000.023.2286553
ACGCAGA380150.023.0695697
CATGGGG192300.022.4616664
ATGGGGG122250.022.4530585
Produced by FastQC (version 0.11.5)