FastQCFastQC Report
Mon 6 Jun 2016
ERR523149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8796845
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343460.39043543452226337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268210.30489340212314753No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT173410.1971274928681817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153470.17446027524640936No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103210.1173261550021627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG128750.045.295122
ACCTGGG140800.039.8821563
GTACCTG151550.039.1316951
TATAACG9450.038.8185922
TAACGCA10750.030.62384
ATAACGC12700.028.5143813
CCTGGGG136450.025.9876694
GTACATG330950.023.219841
GTATCAA524700.022.4436151
TACATGG340600.022.2309572
ACATGGG335000.021.6900543
CATGGGG202350.021.1498724
GAGTACT292950.020.64904812-13
GTATAAC27100.020.477751
GGTATCA400800.020.3348221
TCAACGC579600.020.1392334
CAACGCA584700.019.9388735
ATCAACG588100.019.7845173
AACGCAG599300.019.539626
TATCAAC600300.019.413772
Produced by FastQC (version 0.11.5)