FastQCFastQC Report
Mon 6 Jun 2016
ERR523149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8796845
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325850.37041689378407827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276730.3145786927017584No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT179440.2039822231720577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156180.17754092518397221No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88440.10053604445684788No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG125150.045.1811562
ACCTGGG136900.040.1009453
GTACCTG145800.039.4839551
TATAACG10250.032.5579532
CCTGGGG125900.029.0075134
TAACGCA12300.028.277554
ATAACGC12600.027.6042753
GTATCAA522100.022.2324941
GTACATG327600.022.0948721
GGTATCA398400.021.6745471
TACATGG334350.021.2977582
CATGGGG193350.020.9545464
GAGTACT307500.020.8014512-13
TATCACG9300.020.7216112
ACATGGG326050.020.7151343
ATGGGGG117850.020.4199925
TCAACGC583900.019.6572324
CAACGCA590000.019.4858635
CTGGGGG81200.019.4490535
ATCAACG592350.019.3688833
Produced by FastQC (version 0.11.5)