FastQCFastQC Report
Mon 6 Jun 2016
ERR523148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9692446
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289210.29838701190597294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245780.2535789211515855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133360.1375916873821118No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG101000.029.8007432
GGTATCA276900.029.3311371
GTATCAA398850.029.0411821
ATGGGGG208500.028.5767045
GTACATG429050.028.2137071
TACATGG438600.027.3211692
CATGGGG307550.027.1268654
TGGGGGG208800.026.1815686
ACATGGG445250.026.004613
ACCTGGG114150.025.9555873
TCAACGC460400.024.8115544
CAACGCA462450.024.649575
ATCAACG467850.024.4368113
TATCAAC469400.024.406342
AACGCAG474950.024.1681966
CGTATCA33650.022.20305494
ACGCAGA530000.021.559117
GAGTACT245700.021.49185812-13
CGCAGAG546300.020.9242388
GTACTTT263550.019.77805314-15
Produced by FastQC (version 0.11.5)