FastQCFastQC Report
Mon 6 Jun 2016
ERR523148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9692446
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281850.2907934694709674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248920.2568185574621721No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT197710.20398359712295533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134340.13860278406503374No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT105520.10886828773665595RNA PCR Primer, Index 33 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA286100.030.4111271
GTATCAA408950.028.5551951
TACCTGG95350.027.901662
GTACATG441500.026.8653811
TACATGG451050.026.0629372
ATGGGGG205450.025.7157365
TATAACG7500.025.695492
CATGGGG308400.025.665834
ACATGGG452100.025.0663053
TGGGGGG196750.024.6281176
TCAACGC473650.024.4418744
CAACGCA479000.024.1498765
ATCAACG481450.024.0165983
TATCAAC488100.023.757172
AACGCAG491350.023.5974716
ACCTGGG110550.023.1720983
TAACGCA9000.022.9793514
GAGTACT253800.021.15656312-13
ACGCAGA552600.020.9229417
ATAACGC9800.020.6238613
Produced by FastQC (version 0.11.5)