FastQCFastQC Report
Mon 6 Jun 2016
ERR523147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6424849
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206900.3220309146565156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165110.25698658443178973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95410.1485015445499186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104550.049.69271
GTATCAA182300.039.7282521
TACCTGG69950.038.7110252
GTACCTG83550.033.2317431
TCAACGC218100.032.62664
ATCAACG221850.031.9928573
CAACGCA223700.031.7018185
TATCAAC227500.031.3475842
ACCTGGG84150.031.1126173
AACGCAG233050.030.7024366
GTACATG209800.029.249581
TACATGG215000.027.791182
ACGCAGA264000.027.103047
ACATGGG224800.025.9484233
CGCAGAG275750.025.9118548
TATCACG5500.024.7876422
CATGGGG162150.024.2610784
GCAGAGT305600.022.9806589
CCTGGGG85400.022.1243134
ATGGGGG94550.022.118695
Produced by FastQC (version 0.11.5)