FastQCFastQC Report
Mon 6 Jun 2016
ERR523147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6424849
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187460.2917733942073969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158980.2474455041667127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94130.14650927982898898No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104200.048.5360831
GTATCAA181050.040.3549581
TACCTGG68750.039.6112172
TATAACG5850.032.964032
TCAACGC222600.032.4684374
GTACCTG87050.031.8865261
CAACGCA227050.031.8527815
ATCAACG227300.031.7156093
ACCTGGG85450.031.073023
TATCAAC234550.030.921552
AACGCAG237250.030.542296
TATCACG6900.027.2661112
ACGCAGA271200.026.684227
GTACATG209250.025.7208041
CGCAGAG282750.025.5518978
TACATGG216650.024.445112
TAACGCA8400.023.496344
ACATGGG220250.023.0225093
GCAGAGT320550.022.2308149
CATGGGG159050.021.6571124
Produced by FastQC (version 0.11.5)