FastQCFastQC Report
Mon 6 Jun 2016
ERR523146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14031282
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT517380.3687332347821104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411980.2936153660086085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223720.1594437343644009No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT184150.13124246237799225No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG169850.042.279782
GTACCTG206900.035.2758981
ACCTGGG202400.034.6207583
TAACGCA15700.027.8575274
GTATCAA684300.027.7433591
GGTATCA481200.026.1814631
TATAACG16050.025.1989632
GTACATG515800.024.9357721
TCAACGC776250.024.1124154
CAACGCA784250.023.8471815
TACATGG526450.023.815642
ATCAACG786150.023.7849243
TATCAAC797800.023.490572
AACGCAG806900.023.3144976
ATAACGC18800.023.264083
ACATGGG524900.022.9542623
CCTGGGG187200.022.4590244
CATGGGG315750.021.6858884
ACGCAGA889550.021.0620927
GAGTACT446300.020.98347312-13
Produced by FastQC (version 0.11.5)