FastQCFastQC Report
Mon 6 Jun 2016
ERR523146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14031282
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489880.34913417034879635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414630.2955040031267278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224940.16031322013198793No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT186570.13296718004812388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG172100.040.9961472
GTACCTG209850.034.4509121
ACCTGGG203050.033.773453
TATAACG14600.031.5512392
ATAACGC17300.027.9842833
TAACGCA17000.027.6486664
GGTATCA513550.026.359911
GTATCAA705600.025.3248331
GTACATG532550.023.0788881
CCTGGGG180650.022.6362444
TACATGG542350.022.2565882
TCAACGC796750.021.9985164
CAACGCA807650.021.7137285
ATCAACG809750.021.6801743
ACATGGG535650.021.507273
TATCAAC827950.021.3578682
AACGCAG834250.021.1711736
GAGTACT450200.020.4140412-13
CATGGGG312050.020.1687724
ACGCAGA922350.019.1338887
Produced by FastQC (version 0.11.5)