FastQCFastQC Report
Mon 6 Jun 2016
ERR523145_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523145_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6204109
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189830.30597463713161716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152440.24570812666250705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83870.1351846010442434No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG87400.049.3435022
ACCTGGG97700.042.841093
TATAACG5900.035.0729832
GTACCTG134400.032.402371
CCTGGGG88200.032.205754
GGTATCA166750.031.2492051
GTATCAA241150.029.2529091
TAACGCA6500.028.9408784
ATAACGC6550.028.7199543
GTACATG227450.027.2931121
TACATGG231050.026.4001312
ACATGGG231450.025.0739653
CATGGGG153250.025.0104874
ATGGGGG104950.024.8689865
TCAACGC280900.024.8119854
ATCAACG282400.024.5636223
CAACGCA283850.024.553135
TATCAAC286100.024.4271642
AACGCAG291100.024.044816
TGGGGGG120000.022.877076
Produced by FastQC (version 0.11.5)