FastQCFastQC Report
Mon 6 Jun 2016
ERR523145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6204109
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT278580.44902499295225146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175500.2828770416509445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153810.2479163406058791No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT142760.23010556390933817RNA PCR Primer, Index 23 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80250.12934975836175672No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT76470.12325702208004405RNA PCR Primer, Index 23 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG84750.051.3596422
ACCTGGG94450.045.0871163
GTACCTG129950.033.8035961
CCTGGGG88600.033.687034
GGTATCA159800.032.4043161
TATAACG5950.031.6004832
TAACGCA6200.029.5662084
GTATCAA233450.029.5346891
TGGGGGG100600.028.7793486
CTGGGGG56150.026.7038655
ATAACGC7300.026.3989813
ATGGGGG97800.025.7607865
GTACATG221250.025.7426471
TCAACGC268350.025.2572674
CAACGCA270700.025.0212445
ATCAACG271750.024.8895723
TATCAAC275850.024.6062222
TACATGG228950.024.5140112
CATGGGG150450.024.3682924
AACGCAG280900.024.2278586
Produced by FastQC (version 0.11.5)