FastQCFastQC Report
Mon 6 Jun 2016
ERR523144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6642948
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217710.3277310013566266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174730.263030811019445No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT155200.23363121313007418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97490.14675713252610137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG96550.050.32052
ACCTGGG107650.043.646723
GTACCTG128100.038.403691
TATAACG7800.035.575772
TAACGCA8550.031.9050034
CCTGGGG101800.030.8621084
ATAACGC10000.028.2196373
GTACATG241300.025.825511
TACATGG246700.024.7458532
TATCACG5550.023.7280452
ACATGGG248600.023.440693
CATGGGG156850.023.178854
ATGGGGG96900.022.2276125
CTGGGGG66200.021.3825535
TGGGGGG105800.021.0155126
GAGTACT191900.020.40159812-13
GTATCAA397550.020.2654361
GTACGTG11750.019.2129361
TCAACGC433400.018.3940644
GGTATCA312600.018.2800621
Produced by FastQC (version 0.11.5)