FastQCFastQC Report
Mon 6 Jun 2016
ERR523144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6642948
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196110.2952153170550183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164800.2480826283752334No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT152200.22911514586596193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93540.1408109772950202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG100450.049.041832
ACCTGGG107650.044.5807573
GTACCTG131500.037.9132081
TAACGCA7600.032.1606984
TATAACG7350.031.9770242
CCTGGGG100700.031.5072174
ATAACGC9450.029.8438723
GTACATG228800.024.5858731
CTGGGGG62800.023.6521025
TACATGG237850.023.300542
CATGGGG145000.022.3026354
ACATGGG236600.022.2504623
TATCACG5600.021.8243182
ATGGGGG90300.021.238055
TGGGGGG93100.020.8495586
GTATCAA377550.019.4214691
GAGTACT185400.019.27848612-13
GGTATCA297550.018.4784261
GTATAAC20800.018.3160631
TACCGGG8000.018.2149122
Produced by FastQC (version 0.11.5)