FastQCFastQC Report
Mon 6 Jun 2016
ERR523143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15546376
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1984811.2767026862080268No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA792660.5098680232614984No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT533810.3433661967264911No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT525540.3380466290021546No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC486060.3126516430581635No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC473900.30482988446953807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449610.2892056643940684No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA413250.2658175770353168No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG398280.2561883232465238No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG361510.2325365088300965No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA312440.20097288268339839No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253250.16289970086919292No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA235300.1513536016368059No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG233370.15011215475555204No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC232230.14937886488786842No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG229810.14782223201085576No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT216670.13937010143071285No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG215440.13857892025768578No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT206250.13266757474539403No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG203930.13117526554098524No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG203410.13084078244344532No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT203400.13083435007618496No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT197590.1270971446979026No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA188270.12110217841122586No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA185290.1191853329676318No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG185210.11913387402954875No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC183450.1180017773917214No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC182920.1176608619269211No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT182820.11759653825431726No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG177600.11423884254439748No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC176920.11380144157069146No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT174990.1125599946894376No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT170640.1097619149311711No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC168780.1085654946207399No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC164180.10560660568096385No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT163190.10496980132218596No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG160130.10300149694050884No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG156330.10055719738156338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT22200.045.1301651
CGTCAGA2750.042.75092
ACATATA55300.038.7773062
CTTATGT10250.038.0795822
CATATAG59100.037.7170033
GACATAT62900.034.174791
GCGTCAG3800.033.4210781
TGGGTAT37450.031.1204816
GGCATTC34550.029.3797387
CATGGGT82050.028.7691234
CATTCCG35500.028.5912159
TATCACG2151.8189894E-1228.4343192
GGATATG88900.028.3068981
AAAGACA22100.028.0855644
CTTTAGG36950.027.490162
GGGATGC26900.027.2530147
GGATGCC26950.027.0266828
ATGTATT14450.027.0178361
GGATGAC77100.026.4517968
ACTCCCG43900.025.7987676
Produced by FastQC (version 0.11.5)