FastQCFastQC Report
Mon 6 Jun 2016
ERR523143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15546376
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1913101.2305761805838222No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA744420.47883828359741204No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT499800.3214897156739294No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC496370.3192834137036181No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC469270.3018516984279809No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441570.2840340411167207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407870.2623569634492309No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA395150.254174992294024No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG375960.24183127952134956No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG354370.22794379860618322No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA309950.19937122323556308No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC291940.18778652979961374No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA241670.1554510195816697No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG239570.15410022245698934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233320.15007999291925012No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG219820.14139629711773344No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC217230.1397303139972943No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG215930.13889410625344453No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG214150.1377491448810964No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT210210.1352147921805056No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT208590.1341727486843236No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG195740.12590715675473177No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC194750.12527035239595388No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG194700.12523819055965196No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT193280.12432479440867762No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT189730.12204130403124175No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA189290.1217582798717849No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG186580.12001510834422119No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT181840.11696616626279976No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA179220.1152808860405795No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC175230.11271437150368678No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT173960.11189746086161817No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC172120.11071390528570775No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT170260.10951748497527655No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT169510.10903505743074784No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC163610.10523996074712204No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC162720.10466748006094796No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA161220.10370262497189056No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG160200.10304652351133152No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC155780.10020341718224235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT20950.045.11091
CTTATGT10500.039.837182
ACATATA53250.038.6582682
CATATAG57800.036.1803323
GGGCACG1554.1836756E-1133.3502127
GACATAT62750.032.9692041
ATGTATT13750.031.8016591
TGGGTAT34400.031.5566676
GGCATTC33900.030.4972427
CATTCCG35100.029.595729
TGTACCG1154.2052725E-628.6048185
CTTTAGG34900.028.1454832
TACCTGG45450.028.1269652
GGATATG84000.027.259581
CTCCCGC41600.027.1116227
CCTTTAG36750.026.99571
ACCTGGG46400.026.6365593
TATAACG1807.516064E-926.1105082
CATGGGT80000.026.0228064
GTACCCG2008.112693E-1025.860221
Produced by FastQC (version 0.11.5)