FastQCFastQC Report
Mon 6 Jun 2016
ERR523142_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523142_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12091132
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT468700.3876394699851098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371440.30720035146419705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245180.20277671271804826No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191010.15797528304215022No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG220350.055.5239562
GTACCTG250950.049.233821
ACCTGGG252550.047.7193573
CCTGGGG233100.035.8223764
GGTATCA339050.030.6701341
GTATCAA505650.030.3499341
TATAACG11650.028.6561992
TCAACGC594700.025.496794
CTGGGGG136700.025.3820765
ATCAACG604500.025.0623953
CAACGCA604150.025.058235
GTACATG518250.024.9474491
TATCAAC611100.024.852862
AACGCAG624750.024.4317446
GTACCCG24200.023.9045431
TACATGG534850.023.5607412
TATCACG11450.023.4075262
CATGGGG298900.023.374734
ACATGGG532750.022.9832213
ACGCAGA688000.022.0487697
Produced by FastQC (version 0.11.5)