FastQCFastQC Report
Mon 6 Jun 2016
ERR523142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12091132
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT425500.3519108053737235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365030.3018989454419983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238790.19749184774428066No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173210.14325374993838458No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG222200.057.107622
GTACCTG253250.050.517521
ACCTGGG257900.048.5416033
CCTGGGG225900.036.7371674
GGTATCA348550.030.3650041
TATAACG12150.029.0107732
GTATCAA503150.028.099521
CTGGGGG134200.026.7529725
TATCACG12350.024.3549562
TCAACGC591600.023.4725634
CAACGCA599750.023.1457615
ATCAACG602600.023.0596853
GTACATG516000.022.889321
TATCAAC613800.022.8096122
AACGCAG618650.022.6132626
CATGGGG297150.021.9505274
TAACGCA16300.021.9107484
ATAACGC17350.021.6681443
TACATGG538200.021.5252172
GTACCCG24150.021.2213151
Produced by FastQC (version 0.11.5)