FastQCFastQC Report
Mon 6 Jun 2016
ERR523141_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523141_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7028584
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT550220.7828319331461359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230260.32760510509656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184220.26210115721744237No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT161190.22933495566105488No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC128930.1834366637718209No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC111730.15896516282653803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103940.14788184931701748No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA95400.13573146454534796No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC71070.10111567280123564No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG264950.026.2635571
TACATGG268400.025.4504472
ACATGGG274150.024.1292043
CATGGGG185500.022.200844
GAGTACT194550.021.11806312-13
ATGGGGG109550.020.3849225
GTACTTT207800.019.5565114-15
AGAGTAC322750.018.81229210-11
AGTACTT209200.017.50571812-13
TACCTGG49400.016.8445192
GAGTACA207900.016.6222041
ACTTTTT254250.016.23322116-17
AGTACAT201700.015.8494922
CATGGGA96050.015.8093624
GTATAGC24550.015.5127671
GTGTAGC36550.015.4365281
AATACCG5800.015.4011615
GTATAGG19550.015.3917841
GTATAAT30850.015.2405481
TGGGGGG105000.015.0781846
Produced by FastQC (version 0.11.5)