FastQCFastQC Report
Mon 6 Jun 2016
ERR523141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7028584
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT563050.8010859655372973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209210.2976559716722458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176940.2517434521661831No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT162510.2312130010824371No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC135960.19343867840236384No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC118990.16929441264414No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA98960.14079649613634837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98460.14008511529491574No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG95260.13553227790974684No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA86300.12278433323127388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG261000.026.1976591
TACATGG265150.025.5249732
ACATGGG265400.024.7900983
CATGGGG180700.022.4961974
GAGTACT194750.020.8337212-13
ATGGGGG106200.019.5588235
GTACTTT210600.019.0729414-15
AGAGTAC318850.018.77765510-11
AGTACTT205650.018.09487712-13
TACCTGG48650.017.0995562
GTATAGG21100.016.2696781
GAGTACA208100.016.0896471
ACTTTTT256950.015.73011416-17
CATGGGA97750.015.5287664
AGTACAT203050.015.25375652
TGGGGGG97500.015.1345676
TACTTTT244000.015.13283414-15
CATGGGT42850.014.6962024
GTACCTG61650.014.5693051
GTATATG28000.014.4437171
Produced by FastQC (version 0.11.5)