FastQCFastQC Report
Mon 6 Jun 2016
ERR523140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6098377
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT629661.032504222024975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229370.3761164650857105No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT182330.2989811879455796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179210.29386507262506073No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC138610.22728998223625727No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC123410.2023653178542422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106630.1748498002009387No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA101200.1659457918065741No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG100780.1652570839749658No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA93610.15349985742108105No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC82060.13456039205185247No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG246800.027.5294721
TACATGG250550.026.7604352
ACATGGG254000.025.41673
CATGGGG158450.024.835284
TACCTGG44100.023.6691382
ATGGGGG94350.023.618815
GAGTACT193400.021.4007112-13
GTACTTT211050.019.4114414-15
AGAGTAC310900.019.1277810-11
GTACCTG56450.018.9076291
AGTACTT202050.018.41514212-13
TAACGCA5900.017.5309934
TATCACG5500.017.0976092
TGGGGGG98950.016.8071656
GAGTACA189400.016.6826291
CATGGGA92300.016.1979964
ACCTGGG60100.016.1948993
TATAACG4651.4551915E-1116.1783832
GAGTACG7050.016.0065351
ACTTTTT259750.015.95217916-17
Produced by FastQC (version 0.11.5)