FastQCFastQC Report
Mon 6 Jun 2016
ERR523140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6098377
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT635831.0426216680274112No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210560.34527219291296685No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT177850.29163497107509095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176620.2896180409968095No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC148580.24363859433419746No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC128250.21030185572325227No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG100690.16510950372533545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100580.16492912786467612No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA96060.15751731977212954No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC83600.13708565410108295No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG241250.026.7073611
TACATGG247450.025.584262
TACCTGG45950.024.8549862
ACATGGG248550.024.617083
CATGGGG154150.022.5593994
ATGGGGG88850.022.0555535
GAGTACT193500.020.81199312-13
GTACCTG56500.020.7266251
GTACTTT207400.019.21989614-15
AGAGTAC311900.018.80567410-11
ACCTGGG59900.018.436583
AGTACTT205600.017.8491312-13
TATCACG5850.017.6750052
TGGGGGG89500.017.1697526
CATGGGA95600.016.270824
GAGTACA191650.016.1961881
GAGTACG8150.016.1576481
ACTTTTT252900.015.86057616-17
AGTACAT186600.015.4901712
GTATTAG20100.015.4427831
Produced by FastQC (version 0.11.5)