FastQCFastQC Report
Mon 6 Jun 2016
ERR523139_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523139_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4094319
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134140.32762469167644237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113860.27809264495511954No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT68040.16618148219520756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61570.15037909845324704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG45400.040.090232
ACCTGGG51000.034.4893383
GTACCTG67600.027.5503181
TATAACG4300.027.3435552
GTACATG150400.024.5158161
TACATGG152350.023.7392652
CCTGGGG53200.023.603864
GTATCAA209700.022.7414231
ACATGGG154650.022.5651053
CATGGGG102300.022.4810524
GGTATCA160600.022.314521
ATGGGGG59000.021.5220975
ATAACGC5400.020.9026283
GAGTACT116750.020.20893912-13
GTACACG7550.019.933391
TCAACGC237050.019.9194414
CAACGCA237750.019.8405275
ATCAACG240000.019.5962143
CGCCGTA20000.019.50208794
AACGCAG243650.019.4661396
Produced by FastQC (version 0.11.5)