FastQCFastQC Report
Mon 6 Jun 2016
ERR523139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4094319
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126730.309526443835959No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113780.2778972522658835No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT101350.24753811317584193No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69180.1689658280168204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60000.14654451692699078No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT51560.1259305882125941RNA PCR Primer, Index 33 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG46050.037.1546752
ACCTGGG51450.032.6151433
TATAACG3050.029.2816492
GTACCTG70500.024.5526071
GTACATG141700.023.8006171
TACATGG144650.023.13682
CATGGGG90750.023.1006764
CCTGGGG54350.022.7453524
ATAACGC3950.022.6096043
GGTATCA164450.022.3958111
ACATGGG145250.022.070123
ATGGGGG55200.021.7993055
GTATCAA212900.021.6736621
GAGTACT115750.020.91011212-13
TATCACG3202.0008883E-1120.5645792
TGGGGGG51000.020.2745786
TAACGCA4450.020.0691994
TCAACGC236750.019.2186034
CAACGCA237550.019.114545
ATCAACG240600.018.989223
Produced by FastQC (version 0.11.5)