FastQCFastQC Report
Mon 6 Jun 2016
ERR523138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10307140
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272640.2645156658394084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218390.21188224861600793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120890.11728762779975822No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164400.047.26021
GTATCAA285650.039.44031
TCAACGC339250.032.455464
CAACGCA345050.031.853575
ATCAACG346400.031.801283
TATCAAC358450.031.1300872
AACGCAG358400.030.7393026
ACGCAGA409800.026.7807147
GTACATG352050.026.1332071
CGCAGAG424350.025.8284998
TACATGG363150.024.8380322
ACATGGG374600.023.4731243
GCAGAGT473700.022.8090789
CATGGGG258250.022.0727164
GAGTACT252650.020.56589512-13
CAGAGTA458800.019.7610410-11
AGAGTAC411050.019.65094410-11
GTACTTT272600.018.88942114-15
ATGGGGG139750.018.6616975
AGTACTT275350.017.52254312-13
Produced by FastQC (version 0.11.5)