FastQCFastQC Report
Mon 6 Jun 2016
ERR523138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10307140
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243280.23603055745822799No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210450.20417885077722822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115580.1121358592199194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167300.045.50391
GTATCAA278300.037.802861
TCAACGC337650.030.492984
CAACGCA343700.029.9562265
ATCAACG344900.029.8937453
TATCAAC354050.029.3053342
AACGCAG359900.028.6596326
GTACATG343050.025.7986761
ACGCAGA413200.024.9172447
TACATGG348850.024.794122
CGCAGAG433050.023.7372178
ACATGGG356300.023.2131333
GCAGAGT488600.020.7882659
CATGGGG245900.020.5052684
GAGTACT264950.019.70081712-13
AGAGTAC419950.019.16168410-11
CAGAGTA469450.018.52794510-11
GTACTTT283300.018.24029714-15
GTGGTAT102400.017.4602181
TATACCG7900.017.2502425
Produced by FastQC (version 0.11.5)