FastQCFastQC Report
Mon 6 Jun 2016
ERR523137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524086
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG19300.045.5369952
ACCTGGG24850.034.989663
GTATCAA22300.033.7394521
TGGGGGG25150.033.4411286
GTACATG56500.033.1251261
TACATGG57700.031.9293672
GTACCTG28500.031.5145721
TATAACG1351.328408E-831.332052
TACCGTA1351.3316821E-831.323817
GGTATCA15900.030.7580741
ACATGGG59650.030.1774623
CATGGGG47100.029.2337114
ATGGGGG29000.028.84525
CAACGCA25050.028.7035245
TCAACGC24950.028.6292694
TACACGG3500.028.1988452
GTACACG4050.027.866291
TATCAAC26600.027.2094122
ATCAACG26800.026.6565023
AACGCAG27400.026.4080316
Produced by FastQC (version 0.11.5)